The protein analyzer is named after its functional properties and is an instrument for professional measurement of sample proteins. In fact, it also has a name that everyone is more familiar with, that is, the nitrogen analyzer. In general, the determination of a sample protein by a protein analyzer is based on the classical Kjeldahl method, so the measurement principle is actually the Kjeldahl method. Compared with the traditional measurement method, using a protein analyzer to determine the sample protein is safer, more efficient, accurate, and simple, ensuring the authenticity of the test results and the safety of the operator. So what is the detailed principle of the protein analyzer to determine the sample protein?
The answer to this question is actually to explain what the Kjeldahl principle is. Since protein is a nitrogen-containing organic compound, in the process of determining the protein of a sample using a protein analyzer, we only need to heat and digest the sample, such as food, food, feed, etc., with sulfuric acid and a catalyst to decompose the protein and decompose the ammonia. Combined with sulfuric acid to produce ammonium sulfate. Then alkalized distillation to ammonia free, absorbed with boric acid and then titrated with sulfuric acid or hydrochloric acid standard solution, according to the consumption of acid multiplied by the conversion factor, which is the protein content.
It can be seen from the principle that the use of a protein analyzer to determine the sample protein is actually a relatively complicated chemical reaction process and requires the use of many chemical reagents. Therefore, there is a certain degree of danger, and the operation process is also very It is cumbersome and requires high level of professionalism for the operator. Therefore, it has been cumbersome to measure the protein content of the sample in the past. However, in recent years, with the wide use of protein analyzers, the determination of protein content of samples has become more and more simple. Especially with the development of science and technology, the degree of automation of protein analyzers is continuously improving, and many dangerous operations are now performed. All can be completed by themselves, and the protein analyzer also has the function of safety protection. Therefore, the use of a protein analyzer to measure the sample protein is the most ideal measurement scheme.
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